沙葱黄酮对绵羊外周血淋巴细胞增殖及IL-2、IL-4、IFN-γ mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2015.06.024

Abstract

Abstract:

In order to study the action mechanism of Allium Mongolicum Regel Flavonoids(AMF) on immunological enhancement activity，the effects of AMF on lymphocyte proliferation and mRNA expression of IL-2，IL-4 and IFN-γ were determined by MTT method and real-time fluorescent quantitation PCR assay.The peripheral blood lymphocyte of sheep was used in this study.The results showed that AMF could significantly enhance lymphocyte proliferation.AMF could significantly up-regulate mRNA expression of IFN-γ，down-regulate mRNA expression of IL-4，and have no significantly effect on IL-2 mRNA expression.The result indicate that AMF has immunoregulatory activity and could enhance lymphocyte proliferation.

CLC Number:

S826
S813.3

SA Ru-li，MU Qi-er，WANG Cui-fang，BAO Ling-ling，AO Chang-jin，WANG Si-zhen. Effect of Allium Mongolicum Regel Flavonoids on Lymphocyte Proliferation，mRNA Expression of IL-2，IL-4， IFN-γ in Peripheral Blood Lymphocyte of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1063-1070.

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Effect of Allium Mongolicum Regel Flavonoids on Lymphocyte Proliferation，mRNA Expression of IL-2，IL-4， IFN-γ in Peripheral Blood Lymphocyte of Sheep

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